Mathematisch-Naturwissenschaftliche Fakultät

Institut für Biowissenschaften

Fachgebiet: Mikrobiologie

Betreuer: Prof. Dr. Hubert Bahl

M. Sc. Miriam Mann
(e-mail: )

Metabolic Engineering of Clostridium acetobutylicum: Increase of butanol production with the help of homologous gene expression.

The industrially important bacterium Clostridium acetobutylicum is characterized by a biphasic fermentation metabolism: at the beginning of growth it produces the acids acetate und butyrate. Following the acids are converted to the neutral solvents acetone, ethanol and butanol.

The production of butanol should be increased with the help of homologous gene expression.

The homologous co-expression of the chaperons GrpE and HtpG result in a significant increased resistant to butanol. An increased production of butanol was not possible.

The homologous co-expression of the central key enzymes from C. acetobutylicum fermentation metabolism results in an increased solvent yield.

AS-substitution (R133G, H156N und G222V) from the Thiolase-gene of C. acetobutylicum results in a phenotype with increased butanol production.