The eutrophication of aquatic systems rises by an increased anthropogenic influence. Restoration processes incorporates the element of macrophytes, especially that of Characeae, actively. However, the estimate of the potential of the exploitation of native diaspores requires the determination of seed bank composition and vitality of oospores. Multidimensional analyses have shown an effect on the morphology by the sucrose concentration, depending on the salinity of the habitat. The primary dormancy of Chara oospores can be broken both by the influence of cold stress and dehydration. In respect of germination induction the nutrient and light availability were identified as influence factors. The determination of the vitality of oospores can be effected by means of the detection of dehydrogenases by 2,3,5-Triphenyltetrazoliumchloride.