Mathematisch-Naturwissenschaftliche Fakultät

Institut für Chemie

Fachgebiet: Chemie

Betreuer: Prof. Dr. Udo Kragl

M. Sc. Manuel Gronbach
(e-mail: )

Characterization of the ingredients of plant extracts, examination of their ER-stress reducing activity and application of sublimation to fruit powders to identify marker substances and to isolate natural compounds

In the course of this work, extracts of various plant organs were prepared and their composition was analyzed. Thereby some ingredients of the sea buckthorn berry could be isolated and characterized. In addition to already known ingredients, substances not yet described for sea buckthorn in the literature were obtained. The whole extracts of the investigated plant organs as well as fractions and isolated compounds of the sea buckthorn berry were also tested in cell biological studies for their ER-stress reducing activity.

Additionally, the direct sublimation of substances from fruit powders and dry extracts was investigated. By analyzing the sublimates of different sea buckthorn varieties, marker substances for sea buckthorn berries could be determined. A comparison with other fruit powder sublimates also enabled the determination of specific marker substances of sea buckthorn. The sublimates of the dry extracts of the sea buckthorn berry were also checked for differences in their composition. Depending on the extraction agent used, specific marker substances were found in the sublimates of the dry extracts. In a last step the sublimate of a sea buckthorn berry powder was separated chromatographically. The resulting fractions were analyzed by HPLC-ESI-MS and NMR spectroscopy and some of the ingredients were characterized. In this way, a substance not previously described for sea buckthorn could be isolated for the first time.